Composite

Part:BBa_K4192113:Design

Designed by: Lehan Dong   Group: iGEM22_CAU_China   (2022-09-29)


Plac-RBS-cdg


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 468
    Illegal BamHI site found at 459
    Illegal XhoI site found at 557
    Illegal XhoI site found at 1001
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 392
    Illegal AgeI site found at 959
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 410
    Illegal BsaI.rc site found at 977


Design Notes

It's hard to work alone. It is better to use together with other regulatory factor genes


Source

Plac comes from plasmid pBBR1MCS-2. The rbs sequence (BBa_K4192000) is synthetic. The cdg comes from the genome of Pseudomonas fluorescens 2P24.

References